マウス膣腔内接種法によるHaemophilus influenzaeを中心とした混合感染モデルの実験的研究

Abstract

The intravaginal inoculation model in three-week-old female ddY mice was used for the study of mixed bacterial infection. Neither Escherichia coli nor Enterococcus faecalis alone induced vaginitis : it was cleared within four or five days after inoculation. However，preinoculation with Haemophilus influenzae (serotype b) was able to induce apparent vaginal infection when either of the two bacteria was inoculated intravaginally on the following day. Also in the case of Pseudomonas aeruginosa，only preinoculation with H. influenzae (serotype b) induced apparent vaginitis upon inoculation with P. aeruginosa on the following day. It is speculated that H. influenzae (serotype b) may destroy the mechanical barrier of the vaginal epithelium，possibly thereby inducing a local compromised condition on the mucosal epithelium.