アミノヌクレオシド腎症における腎糸球体および血小板膜陰性荷電の変化

Abstract

In aminonucleoside nephrosis (AN), which can be regarded as an experimental model of minimal change nephrotic syndrome (MCNS), ultrastructural alteration of glomerular anionic sites were studied using polyetyleneimine (PEI) as a cationic probe, and correlated with the change of platelet membrane negative charge. In control rats, PEI was seen in epithelial cell surface and in both lamina rara externa and lamina rara interna. On the other hand, AN rats, showed a loss of polyanion on the epithelial surface associated with foot process fusion and a reduction of anionic sites within glomerular basement membrane. The degree of anionic sites correlated with proteinuria. The negative charge of pletelet membrane also decreased in AN rats and correlated with a loss of glomerular anionic sites. However, there was no remarkable change in platelet morphological findings on electron microscopy. These findings may provide useful information on platelet function in pediatric MCNS.