頭頸部領域を中心としたヒト正常及び腫瘍細胞の三次元培養による組織再構築の研究

Abstract

Three-dimensional culture was performed using gamma-irradiated type Ⅰ collagen gel prepared from rat tail tendon. The morphological outgrowing appearance with in type Ⅰ collagen gel provided a marked difference in accordance with histopathological diagnosis, subsequently suggesting the difference in adhesion ability to type Ⅰ collagen. On the other hand, type Ⅰ collagen may be involved in differentiation-related proteins expressed during in vitro morphogenesis, such as carcinoembryonic antigen for tubulogenesis of the salivary gland. The present experiment system would be useful for studying further the relation between type Ⅰ collagen matrix and metastatic potentiality and/or cellular differentiation.