加熱処理活性化プロトロンビン複合体製剤(APCCs)の凝血学的特性

Abstract

We studied in vitro properties of heat-treated APCCs (Autoplex T and FEIBA TIM4) as compared with a heat-treated PCC (Proplex ST) with special reference to vitamin K dependent factors and F. Ⅷ inhibitor bypassing activity (FEIB-activity). Autoplex and FEIBA were found to contain all of Factors Ⅱ, Ⅶ, Ⅸ and Ⅹ activities equal to or more than those levels in Proplex. While the immunological activity of F. Ⅱ and F. Ⅹ in both APCCs was higher than their functional activity, the functional activity of F. Ⅶ and F. Ⅸ was higher than their immunological activity. With western blot technique, bands of F. Ⅶa, F. Ⅸa and F. Ⅹa were shown in autoplex, FEIBA and Proplex. The content of FEIB-activity in both APCCs was the same as or more than indicated individually on the label. These findings suggest that activation and inactivation of biological activity and some modification of prothrombin family proteins in Autoplex and FEIBA occurred during production and heating procedures, and that both APCCs are probably effective and useful for managing hemophiliacs with inhibitors.