肺癌組織におけるレクチン結合性

Abstract

In order to find a cancer specific pattern of lectin bindings, semiquantitative immunohistochemical analysis using a panel of lectins (DBA, UEA-Ⅰ, RCA-Ⅰ, WGA, ConA, PNA) was conducted on fresh-frozen tissue sections of non-cancerous bronchus, metaplastic bronchus and lung cancer. The following results were obtained : 1) In non-cancerous bronchus, the binding distributions of RCA-Ⅰ, WGA, ConA, and PNA with neuraminidase (PNA•N(+)) were observed in almost all tissue components ; in contrast, those of DBA, DBA•N(+), UEA-Ⅰ, and PNA were localized in bronchial epithelial cells, bronchial glands, and endothelial cells. 2) All the lectins except for PNA bound to the metaplastic bronchial epithelial cells. 3) Almost all the cancerous tissues were positively stained with UEA-Ⅰ, RCA-Ⅰ, WGA, ConA, and PNA•N(+). DBA was found to bind adenocarcinomas preferentially to squamous cell carcinomas and small cell carcinomas. 4) Bindings of DBA and UEA-Ⅰ in lung cancer tended to be correlated with cancer cell differentiation. 5) There were relationships between DBA, UEA-Ⅰ, RCA-Ⅰ, and PNA bindings and clinical staging in lung cancer. 6) DBA and UEA-Ⅰ bindings showed no relationship with ABO blood group status of lung cancer patients. 7) The incidence of the phenotype, PNA, PNA•NC(+)⁺ representing the cryptic PNA binding sites were masked by sialic acids might be correlated with the serum CEA values in squamous cell carcinoma and lymphnodes metastasis. In conclusion, semi-quantitative lectin immunohistochemistry using DBA, UEA-Ⅰ, and PNA contributes to the assessment of various abnormalites of glycoconjugates associated with lung cancer.