Rhodamine123のヒト由来前立腺癌培養細胞の増殖に及ぽす影響に関する研究

Abstract

A lot of novel chemotherapeutic agents have been developed recently, improving the management of urogenital carcinomas. However, effective chemotherapy has not yet been established for advanced renal cell carcinoma and hormone relapsing prostate carcinoma. Most of the chemotherapeutic agents target DNA in tumor cells; therefore, the new agents functioning in different mechanisms to inhibit tumor cell growth are expected for the treatment of these carcinomas. Rhodamine 123 (Rh), a red fluorescent dye, has the property to acummulate more and be retained longer in the mitochondria of malignant transformed cells than in those of normal cells. In this study, the suppressive effect of Rh on the growth of cultured prostate carcinoma cells (PC-3 and LNCaP) was examined in vitro and in vivo. Rh suppressed the growth of PC-3 and LNCaP cells significantly. The accumulation and retention of Rh in PC-3 cells was demonstrated both in vitro and in PC-3 tumor cel1s implanted in nude mice. Methylglyoxal Bis(guanylhydrazone) (MGBG) and 2-deoxy-D-glucose (2DG) suppressed the growth of PC-3 and LNCaP cells when each was administered in combination with Rh ; namely, these agents seem to modulate the suppressing effect of Rh on the growth of tumor cells. These results indicate that Rh has the potential to be an anticancer agent against prostate carcmomas.