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このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10564/3935

タイトル: Molecular and Epidemiological Characteristics of Carbapenemase-Producing Klebsiella pneumoniae Clinical Isolates in Japan.
その他のタイトル: 本邦で分離されたカルバペネマーゼ産生肺炎桿菌の分子遺伝学的解析
著者: Yonekawa, Shinsuke
Mizuno, Tomoki
Nakano, Ryuichi
Nakano, Akiyo
Suzuki, Yuki
Asada, Tomoko
Ishii, Ayako
Kakuta, Naoki
Tsubaki, Kosuke
Mizuno, Sayaka
Ogawa, Miho
Yano, Hisakazu
Kasahara, Kei
Mikasa, Keiichi
キーワード: Klebsiella pneumoniae
carbapenemase
hypervirulent clones
発行日: 2020年10月21日
出版者: American Society for Microbiology
引用: mSphere Vol.5 No.5 Article No.e00490-20 (2020 Oct)
抄録: Carbapenemase-producing Enterobacteriaceae represent a serious public health threat worldwide. Carbapenemase genes, harbored on a transferable plasmid, have been isolated globally with distinct geographical features. Klebsiella pneumoniae, included in Enterobacteriaceae, also produces carbapenemase and often shows hypervirulence. Overlapping carbapenem resistance and hypervirulence in K. pneumoniae have been reported, but such strains have not yet been found in Japan. Here, we screened 104 carbapenemase-producing K. pneumoniae isolates collected from 37 hospitals and outpatient clinics in Japan between September 2014 and July 2015. PCR and DNA sequencing demonstrated IMP-1 in 21 isolates and IMP-6 in 83 isolates, 77 of which coharbored CTX-M-2. Most of the isolates showed low MICs toward imipenem and meropenem but high MICs toward penicillin and cephalosporins. Conjugation experiments with an Escherichia coli J53 recipient showed that most of the plasmids in IMP-6 producers were transferable, whereas only one-half of the plasmids in IMP-1 producers were transferable. PCR-based replicon typing and multiplex PCR identified five isolates belonging to the CG258 non-tonB79 cluster and no isolate belonging to the CG258-tonB79 cluster or sequence type 307 (ST307). Four K1-ST23 isolates, 10 K2-ST65 isolates, and 7 K2-ST86 isolates were detected that harbored virulence genes. The resistance genes in 85 isolates were transferable, but the virulence genes were not transferred. These results demonstrate the acquisition of IMP-type carbapenemase genes and CTX-M-type genes among hypervirulence isolates in Japan, warranting further attention and countermeasures. In this study, we have determined the molecular characteristics and epidemiology of IMP-6 producers that coharbored various CTX-M genes in Japan.IMPORTANCE Carbapenems serve as a last resort for the clinical treatment of multidrug-resistant infections. Therefore, the rapid spread of carbapenemase-producing strains represents a serious public health threat, further limiting antibiotic choices. The current findings of hypervirulent carbapenemase-producing Klebsiella pneumoniae clinical isolates in Japan demonstrate the potential broad spread and transfer of these genes, necessitating close surveillance.
内容記述: 博士(医学)・乙第1509号・令和3年3月15日
Copyright © 2020 Yonekawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license(https://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/10564/3935
ISSN: 23795042
DOI: https://doi.org/10.1128/mSphere.00490-20
学位授与番号: 24601B1509
学位授与年月日: 2021-03-15
学位名: 博士(医学)
学位授与機関: 奈良県立医科大学
出現コレクション:2020年度

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