GINMU >
01 奈良県立医科大学 >
011 医学部 >
0112 紀要 >
01121 Journal of Nara Medical Association >
Vol.50 No.2 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10564/500
|
タイトル: | ハムスターにおける膵細胞増殖能を指標とした膵発癌物質の検索 |
その他のタイトル: | DETECTION OF PANCREATIC DUCTAL CARCINOGENS BY MEASURING DUCTAL CELL PROLIFERATION AS AN INDICATOR IN HAMSTERS |
著者: | 天沼, 利宏 |
キーワード: | hamster pancreas ductal carcinoma carcinogens cell proliferation |
発行日: | 1999年4月30日 |
出版者: | 奈良医学会 |
引用: | Journal of Nara Medical Association Vol.50 No.2 p.74-86 |
抄録: | Since a high incidence of pancreatic ductal carcinomas can be obtained
within only 10 weeks by repeated "augmentation pressure" treatment in a rapid production
model for hamsters, chemically initiated cell populations are believed to have proliferative
advantage. Based on this hypothesis, I studied the 5-bromo-deoxyuridine (BrdU) labeling
index (LI), as an indicator of cell proliferation during "augmentation pressure". I also
studied the effects of several non-pancreatic carcinogens on cell proliferation in this
experimental system to screen their possible contribution to pancreatic carcinogenesis. The
results were as follows :
1) In control groups without initiation, LI of pancreatic ductal cells increased slightly
during augmentation pressure, with a peak being observed 16 days after the study com-
mencement.
2) Among pancreatic ductal carcinogens in hamsters, carcinogenic doses of both N-
nitrosobis(2-oxopropyl)amine or N-nitrosobis(2-hydroxypropyl)-amine clearly amplified
the increase of LI in main pancreatic duct cells. However, N-methyl-N-nitrosourea,
administered below a carcinogenic dose, had no effect.
3) Both azaserine and 4-hydroxyaminoquinoline, pancreatic acinar cell carcinogens, also
amplified the increase of LI in main duct cells.
4) Among non-pancreatic carcinogens, diethylnitrosamine, dimethylnitrosamine, and 3-
amino-1, 4-dimethyl-5H-pyrido(4, 3-b)indole amplified the increase of LI in main duct
cells, while N-methyl-N-nitroso-N'-nitrosoguanidine and benzo(a)pyrene had no effect.
These results suggest that initiated cell populations selectively proliferate during "augmentation pressure" and that quantification of cell proliferation under this experimental
system is a useful marker to detect pancreatic ductal carcinogens within a short period.
Also our data show possible risk of several carcinogens regarding pancreatic ductal
neoplasia through increasing proliferation of target cell populations. |
URI: | http://hdl.handle.net/10564/500 |
ISSN: | 13450069 |
出現コレクション: | Vol.50 No.2
|
このリポジトリに保管されているアイテムは、他に指定されている場合を除き、著作権により保護されています。
|