培養骨髄細胞含浸人工真皮移植による創傷治癒促進効果についての検討

Abstract

[Purpose] Recently, reconstruction of skin defects using artificial dermis composed of an outer layer of silicone and an inner sponge layer of collagen has been developed and is performed clinically. When the artificial dermis is grafted onto a total skin defect, the inner sponge layer spontaneously converts into dermis-like connective tissue. However, 2 or 3 weeks after the application of the artificial dermis, a secondary split -thickness skin graft on the dermis-like tissue is required for skin resurfacing. Until the secondary skin graft, problems of wound infection or tissue fluid leakage persist. In this study, the authors investigated the effect of cultured bone marrow cells on the synthesis of dermis-like tissue using artificial dermis in rats. [Methods] Two rats were sacrificed to harvest bone marrow cells from the femurs, and the cells were cultured for 10 days. Full thickness skin defects (3 cm×4 cm) were made on the backs of 20 male Fisher rats, then the rats were divided into 5 groups. The artificial dermis containing 10^4(10^5, 5×10^5, 10^6)/ml bone marrow cells were grafted on the skin defects of rats in Group 1(2, 3, 4). In Group 5, artificial dermis only was grafted. After 10 days, the grafted artificial dermis was harvested, and histological examination was perfor- med. In each group, mean thickness of dermis-like tissue, which was infiltrated by fibroblasts and capillaries, was measured. [Result] The dermis-like tissue was significantly thicker in Groups 1～4 than in Group 5, and was significantly thickest in Group 2. [Conclusion] Histologically, topical application of bone marrow cells accelerates prolif- eration of fibroblasts and capillaries in artificial dermis. Therefore, this study suggests the usefulness of bone marrow cells combined with artificial dermis for wound healing.