AFP産生胃癌細胞株(FU97)の生物学的特性に関する検討

Abstract

A new cell line, designated as FU97, was established from a 66-year-old gastric cancer patient with a high serum level of α-fetoprotein (AFP). This cell line produced a large amount of AFP in the conditionated medium as measured by radioim- munoassary (RIA), and AFP production in situ was also demonstrated by immunohisto- chemical study. The population doubling time was 95 hours. The cultured cells were positive for tumorigenicity in athymic nude mice and the histological feature of the xenografted tumor was similar to that of the tumor primarily isolated from the patient. The amount of AFP in the xenografted mouse serum, as measured by RIA, increased with tumor growth. In collagen gel matrix, cultured cells grew with medullary architecture, and chemosensitivity test was achievable. FU97 is considered to be an AFP-producing gastric cancer cell line and will provide a useful tool for the study of the production and secretion of AFP from cells, and also to elucidate the biological activities of AFP.